Atorvastatin Alleviates Myocardial Ischemia-Reperfusion Injury via miR-26a-5p/FOXO1

Purpose: Ischemia-reperfusion (I/R) injury exacerbates myocardial cell death (including apoptosis and necrosis), leading to complications such as arrhythmias, stenosis, microvascular obstruction heart failure, it is particularly important seek new strategies mitigate reperfusion injury. In this paper, we will investigate whether atorvastatin can alleviate ischemia-reperfusion verify its molecular mechanism. Methods: We successfully constructed a hypoxia-reperfusion (H/R) H9c2 model transfected miR-26a-5p mimic, inhibitor negative control NC-mimic or NC-inhibitor into cells using transfection kit. The expression of FOXO1 were detected by RT-qPCR assay, the related proteins Western blot viability CCK-8 rate flow cytometry, CK LDH activity in assay kits. targeting relationship between was verified dual luciferase reporter gene assay. Results: MiR-26a-5p decreased H/R-induced increased cells. Atorvastatin alleviated H/R cardiomyocytes most effective at concentration 1 μM. upregulating expression, negatively regulated targeting. Conclusion: regulating miR-26a-5p/FOXO1.